Journal: Oncology Letters

Article Title: Blocking integrin β1 decreases adhesion in chemoresistant urothelial cancer cell lines

doi: 10.3892/ol.2017.6883

Figure Lengend Snippet: Flow cytometry analysis of integrin surface expression. Cells were washed in blocking solution and stained with specific monoclonal antibodies as listed in materials and methods. Mouse IgG1-PE or mouse IgG2a-PE antibodies were used as isotype controls. Fluorescence was analyzed using a FACScan flow cytometer. The relative fluorescence unit values are given in percentage difference to the parental cell lines. Parental cell lines were set as 100%. One of three independent experiments is shown here. *P≤0.05 vs. controls.

Article Snippet: Tissue sections were stained for Integrin β1, (D2E5) Rabbit mAb, Cell Signaling Technology (Waltham, MA, USA), dilution 1:100.

Techniques: Flow Cytometry, Expressing, Blocking Assay, Staining, Fluorescence

Journal: Oncology Letters

Article Title: Blocking integrin β1 decreases adhesion in chemoresistant urothelial cancer cell lines

doi: 10.3892/ol.2017.6883

Figure Lengend Snippet: Expression of integrin β1 in non-malignant urothelium and urothelial cancer. (A) Normal urothelium showed a negative or a weak expression of intergrin β1 only in the basal cell layer. A higher integrin β1 expression was visible in samples of (B) non invasive low grade urothelial cancer, (C) non invasive high grade cancer, and (D) muscle invasive high grade cancer. Immunostaining of 4 representative tissue samples.

Article Snippet: Tissue sections were stained for Integrin β1, (D2E5) Rabbit mAb, Cell Signaling Technology (Waltham, MA, USA), dilution 1:100.

Techniques: Expressing, Immunostaining

Journal: Oncology Letters

Article Title: Blocking integrin β1 decreases adhesion in chemoresistant urothelial cancer cell lines

doi: 10.3892/ol.2017.6883

Figure Lengend Snippet: Influence on adhesion after blocking integrin β1. Bladder cancer cell lines were preincubated for 60 min with a monoclonal anti-integrin β1 blocking antibody (20 mg/l). Controls remained untreated. Cells were then subjected to the adhesion assay as indicated. Untreated parental cells were set as 100%. *P≤0.05 vs. controls.

Article Snippet: Tissue sections were stained for Integrin β1, (D2E5) Rabbit mAb, Cell Signaling Technology (Waltham, MA, USA), dilution 1:100.

Techniques: Blocking Assay, Cell Adhesion Assay

Journal: Oncology Letters

Article Title: Blocking integrin β1 decreases adhesion in chemoresistant urothelial cancer cell lines

doi: 10.3892/ol.2017.6883

Figure Lengend Snippet: Influence on chemotaxis after blocking integrin β1. Bladder cancer cell lines were preincubated for 60 min with a monoclonal anti-integrin β1 blocking antibody (20 mg/l). Controls remained untreated. Cells were then subjected to the chemotaxis assay as indicated. Untreated parental cells were set as 100%. *P≤0.05 vs. controls.

Article Snippet: Tissue sections were stained for Integrin β1, (D2E5) Rabbit mAb, Cell Signaling Technology (Waltham, MA, USA), dilution 1:100.

Techniques: Chemotaxis Assay, Blocking Assay

Journal: Aging (Albany NY)

Article Title: Melatonin regulates mitochondrial dynamics and alleviates neuron damage in prion diseases

doi: 10.18632/aging.103328

Figure Lengend Snippet: Melatonin reduced synapse damage in PrP 106-126 -treated N2a cells. ( A , B ) Protein expression of PSD95 by Western blotting. ( C ) Representative images of mitochondria (Original magnification 600×). ( D ) Quantification of spinophilin. ( E ) Cells with clustered perinuclear mitochondria *P < 0.05, **P < 0.01. All experiments were repeated at least three times.

Article Snippet: The following antibodies were used: anti-cleaved caspase-9 (9509T, CST), anti-cleaved caspase-3 (9664T, CST), anti-Bax (#2772, CST), anti-Bcl2 (#3498, CST), anti-cytochrome c (10093, Proteintech), VDAC rabbit mAb (4661, CST), anti-MFN1 (NBP1-71775, Novus Biologicals), DRP1 rabbit mAb (8570, CST), OPA1 rabbit mAb (80471, CST), anti-FIS1 (D122377-0025, BBI life sciences), anti-MFN2 (12186, Proteintech), anti-beta tubulin (10094, Proteintech), anti-PSD95 (20665, Proteintech), spinophilin rabbit mAb (14136, CST, Boston), HP-goat anti-mouse (ZB-2305, Zsbio, Beijing, China), HP-goat anti-rabbit (ZB-2301, Zsbio, Beijing, China), and Alexa Fluor 594 AffiniPure Goat Anti-Rabbit IgG (H+L) (33112ES60, Yeasen).

Techniques: Expressing, Western Blot